Histone deacetylase is a component of the internal nuclear matrix.

摘要: In chicken immature erythrocytes, approximately 4% of the modifiable histone lysine sites participate in active acetylation. There are two categories actively acetylated H4. Although both at same rate (t1/2 = 12 min), one is to tetraacetylated form and rapidly deacetylated (class 1), other mono- diacetylated forms slowly 2). We show that chromatin distribution class 1 labeled H4 species paralleled transcriptionally DNA sequences. For example, fragments insoluble nuclear material contained 76% 74% Class 2 were found repressed enriched active/competent gene-enriched fragments. The majority deacetylase activity (75-80%) was located with residual material. Further, 40-50% enzyme associated matrices prepared by methods using high salt intermediate/high extraction. Histone solubilized extracting 2-mercaptoethanol, a procedure generates pore-lamina complexes. These results demonstrate component internal matrix.