Fibrin activation of vascular endothelial cells. Induction of IL-8 expression.

摘要: Fibrin deposition and leukocyte accumulation are classic histopathologic hallmarks of acute chronic inflammation. Although both these processes common to inflammatory processes, surprisingly little is known about the interrelationship between fibrin accumulation. We hypothesized that: 1) can regulate inflammation by directly inducing chemotactic factor (LCF) expression from vascular endothelial cells (VEC), 2) this fibrin-induced VEC LCF would enhance recruitment seen in To test hypothesis, we developed an vitro model system situ polymerization on isolated bovine pulmonary artery culture. In initial phase our studies, found that induced a time- dose-dependent migration activity VEC. Checkerboard analysis demonstrated present fibrin-stimulated culture supernatant was truly nature. effort begin characterize activity, protein dependent, not derived fibrin(ogen), 3) had apparent m.w. 6 12 kDa. Our further studies potent stimulus for IL-8 Ag Thus, clearly demonstrate has capability induce general, specifically, These support hypothesis important activator vivo.