In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy.

摘要: In this study we coated a new biocompatible, nanostructured titanium alloy, Ti13Nb13Zr, with thin layer of hydroxyapatite nanocrystals and investigated the response human bone-marrow-derived mesenchymal cells. The coating was realized using slightly supersaturated CaP solution, which provokes fast deposition nanocrystalline hydroxyapatite. A is appreciable on etched Ti13Nb13Zr substrates after just 1.5 h soaking in it reaches thickness 1–2 μm 3 soaking. seems thinner than that deposited Ti6Al4V, examined for comparison, likely because different roughness profiles two alloys, constituted elongated HA nanocrystals, mean length about 100 nm. Mesenchymal stem cells were seeded onto uncoated Ti alloys cultured up to 35 days. Cell morphology, proliferation differentiation evaluated. display good adhesion substrates, whereas presence reduces cell induces MSCs towards phenotypic osteoblastic lineage, agreement increase expression osteopontin, osteonectin collagen type I, evaluated by means rt-PCR. Type I higher MSC culture compared standing more efficient extracellular matrix deposition. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res,