A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors.

摘要: Several methods have been developed for the detection of minimal residual disease (MRD) in B cell tumors. Chromosomal translocations or rearrangement immunoglobulin heavy chain (IgH) and T receptor genes are generally employed. We report a novel PCR method to detect MRD using IgH genes. rearranged variable region (VDJ) were amplified from tumor specimens consensus primers joining Complementarity-determining regions (CDR) identified used generate tumor-specific primers. Two-round amplifications derived CDRs constant performed detection. nested-PCR approach was tested on panel 75 tumors including acute lymphoblastic chronic lymphocytic leukemias, non-Hodgkin’s lymphomas multiple myelomas. A VDJ sequence obtained 62 out cases (83%). Sensitivity DNA cDNA templates 10−5 −6, respectively. This is specific sensitive provides simple, non-radioactive evaluation