Cyanide-induced transition from endogenous carbohydrate to lipid oxidation as indicated by the carbon-13 content of respiratory CO2

摘要: The respiration of thin aerated discs potato tuber tissue rises sigmoidally through 24 h. Aged disc is ostensibly resistant to concentrations cyanide which inhibit the fresh discs. It has been shown that cyanide-resistant does not represent indifference inhibitor, but rather due suppression one respiratory carbon path and evocation another. predominant aged in absence comprises glycolysis linked tricarboxylic acid cycle. mediating cyanide-induced reflects cycle-independent lipid degradation. The substrate at any time was deduced by comparing 13C/12C ratio respired CO2, collected from presence or cyanide, with ratios characterizing endogenous potential metabolites. determination discs, have an reserve, proved possible because relative stable isotopes compounds such as starch are widely different.