Lactobacillus oligofermentans glucose, ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism-induced responses in the early exponential growth phase

摘要: Lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role spoilage is unknown. It belongs to the L. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than hexoses glucose). However, efficient hexose utilization can be induced. The regulation mechanisms carbohydrate catabolism such bacteria have scarcely studied. To address this question, we provided complete genome sequence LMG 22743T generated time course transcriptomes during growth on glucose, ribose xylose. was manually annotated main functional features were examined. confirmed able utilize several maltose, which is, presumably, induced by repeated cultivation glucose vitro. Unexpectedly, beginning exponential phase, glucose- xylose-induced transcriptome responses similar, whereas toward end phase became alike. promoter regions genes simultaneously upregulated both comparison (particularly, genes) found enriched CcpA- binding site. Transcriptionally, no glucose-induced carbon catabolite repression detected. requires initial oxidation, led significant overexpression NAD(P)H re-oxidation genes, upstream contain a motif, highly similar Rex repressor This paper presents second first study catabolism-dependent response for member group. transcriptomic changes detected different carbohydrates differ significantly those facultative lactobacilli. mechanism CcpA regulation, putatively contributing observed similarities between absence stringent control, further studies. Finally, cell redox balance maintenance, terms NAD(P)+/NAD(P)H ratio, predicted regulated transcriptional regulator, supporting previously made inference Rex-regulons members Lactobacillaceae family.