A novel approach to clonality analysis. Alterations in genomic methylation of Epstein-Barr virus transformed lymphocytes.
作者： A.N. Goldfarb , C.E. Litz
DOI: 10.1016/S0344-0338(11)80515-7
关键词: breakpoint cluster region 、 Biology 、 Epstein–Barr virus 、 Genetics 、 Allelic heterogeneity 、 Allele 、 Chronic myelogenous leukemia 、 Methylation 、 Virus 、 Locus (genetics) 、 Molecular biology
摘要: Summary We have studied eight different Epstein-Barr virus transformed cell lines (EBV-LCL) with respect to genomic methylation at a single locus, M-bcr (the major breakpoint cluster region of chronic myelogenous leukemia). Restriction digests the sensitive enzyme, Hpall, illustrated marked differences in patterns between various lines. Some displayed prominent allelic heterogeneity: within each these samples there were numerous M-bcr/Hpall fragments on Southern analysis. Other predominance: limited number predominated. Analysis Immunoglobulin JH rearrangements demonstrated close correlation clonal complexity (i.e. rearranged fragments) and heterogeneity fragments). conclude that analysis locus may offer novel approach clonality determination EBV lymphocytes.
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