Proteomic analysis of seed germination under salt stress in soybeans

摘要: Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop, and its production severely affected by saline soils. Therefore, the response of soybean seeds to salt stress during germination was investigated at both physiological proteomic levels. The salt-tolerant cultivar Lee68 N2899 were exposed 100 mmol/L NaCl until radicle protrusion from seed coat. In cultivars, final percentage not salt, but mean times delayed 0.3 1.0 d, respectively, compared with controls. stress, abscisic acid content increased, gibberellic (GA1+3) isopentenyladenosine decreased. Indole-3-acetic increased in Lee68, remained unchanged N2899. proteins extracted germinated separated using two-dimensional gel electrophoresis (2-DE), followed Coomassie brilliant blue G-250 staining. About 350 protein spots 2-DE gels pH range 3 10 650 4 7 reproducibly resolved, which 18 showed changes abundance as result cultivars. After matrix-assisted laser desorption ionization-time flight-mass spectroscopy (MALDI-TOF-MS) analysis differentially expressed proteins, peptide mass fingerprint searched against UniGene database nine successfully identified. Ferritin 20S proteasome subunit β-6 up-regulated Glyceraldehyde 3-phosphate dehydrogenase, glutathione S-transferase (GST) 9, GST 10, maturation PM36 down-regulated still certain level. However, these present lower levels control under stress. results indicate that might have important roles defense mechanisms germination.