Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles.

摘要: Nanoparticles have unique properties that make them attractive for use in industrial and medical technology industries but can also be harmful to living organisms, making an understanding of their molecular mechanisms action essential. We examined the effect three different sized poly(isobutyl-cyanoacrylate) nanoparticles (iBCA-NPs) on unicellular green alga Chlamydomonas reinhardtii. found exposure iBCA-NPs immediately caused C. reinhardtii display abnormal swimming behaviors. Furthermore, after one hour, most cells had stopped 10%-30% were stained with trypan blue, suggesting these severely impaired plasma membranes. Observation cyto-ultrastructure showed cell walls been damaged many located space between wall membrane, as well inside cytosol some cases. A comparison strains conditions further mortality ratio increased more rapidly absence a wall. Interestingly, over time was essentially identical regardless iBCA-NP size if total surface area same. direct observation trails indicated first trigger contact wall, which is likely accompanied by inactivation or removal adsorbed proteins from surface. Cell overproduction reactive oxygen species, detected readily grown under constant light rather than dark.