Performance of Simplexa Dengue Molecular Assay Compared to Conventional and SYBR Green RT-PCR for Detection of Dengue Infection in Indonesia
作者： R. Tedjo Sasmono , Aryati Aryati , Puspa Wardhani , Benediktus Yohan , Hidayat Trimarsanto
DOI: 10.1371/JOURNAL.PONE.0103815
关键词: Virus isolation 、 Gold standard (test) 、 Microbiology 、 Real-time polymerase chain reaction 、 Serotype 、 Virology 、 Dengue fever 、 Dengue virus 、 Polymerase chain reaction 、 Typing 、 Biology 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Diagnostic tests based on detection of dengue virus (DENV) genome are available with varying sensitivities and specificities. The Simplexa Dengue assay (Focus Diagnostics) is a newly developed real-time RT-PCR method designed to detect serotype DENV simultaneously. To assess the performance assay, we performed comparison conventional SYBR Green patients sera isolated from eight cities across Indonesia, endemic country. A total 184 that were confirmed using NS1 and/or IgM IgG ELISA examined. Using RT-PCR, detected in 53 (28.8%) 81 (44.0%) out sera, respectively. When was employed, rate increased 76.6% (141 samples). tested 40 by isolation as gold standard, yielded 95% sensitivity while reached 97.5% 100%, specificities all methods 100% when 43 non-dengue illness 20 healthy human samples. Altogether, our data showed higher compared field/surveillance setting. In conclusion, offers rapid accurate typing infection suitable for both routine diagnostic surveillance.
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