Mung bean nuclease I. IV. An improved method of preparation
作者： W. Ardelt , M. Laskowski
DOI: 10.1016/S0006-291X(71)80214-0
关键词: Enzyme 、 Potency 、 Mung Bean Nuclease 、 Improved method 、 Chemistry 、 Biotechnology 、 Chromatography 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Summary An eight step procedure leading to a 25,000 fold purification of the enzyme is described. The overall yield 20%, final potency ‡ 3,300. purified preparation approaches homogeneity.
elsevier.com
sci-hub.se 参考文章(7)
Shan-ching Sung, M. Laskowski, A nuclease from mung bean sprouts. Journal of Biological Chemistry. ,vol. 237, pp. 506- 511 ,(1962) , 10.1016/S0021-9258(18)93952-9
Östen Levin, [2] Column chromatography of proteins: Calcium phosphate Methods in Enzymology. ,vol. 5, pp. 27- 32 ,(1962) , 10.1016/S0076-6879(62)05184-8
Hamilton O. Smith, K.W. Welcox, A Restriction enzyme from Hemophilus influenzae: I. Purification and general properties Journal of Molecular Biology. ,vol. 51, pp. 379- 391 ,(1970) , 10.1016/0022-2836(70)90149-X
Paul Hickok Johnson, M. Laskowski, Mung Bean Nuclease I II. RESISTANCE OF DOUBLE STRANDED DEOXYRIBONUCLEIC ACID AND SUSCEPTIBILITY OF REGIONS RICH IN ADENOSINE AND THYMIDINE TO ENZYMATIC HYDROLYSIS Journal of Biological Chemistry. ,vol. 245, pp. 891- 898 ,(1970) , 10.1016/S0021-9258(18)63349-6
Hamilton O. Smith, Thomas J. Kelly, A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition site Journal of Molecular Biology. ,vol. 51, pp. 393- 409 ,(1970) , 10.1016/0022-2836(70)90150-6
A.J. Mikulski, M. Laskowski, Mung Bean Nuclease I III. PURIFICATION PROCEDURE AND (3')-ω-MONOPHOSPHATASE ACTIVITY Journal of Biological Chemistry. ,vol. 245, pp. 5026- 5031 ,(1970) , 10.1016/S0021-9258(18)62813-3
Thomas J. Kelly, Hamilton O. Smith, A restriction enzyme from Hemophilus influenzae. II. Journal of Molecular Biology. ,vol. 51, pp. 393- 409 ,(1970) , 10.1016/0022-2836(70)90150-6