Impacts of reclaimed water irrigation on soil antibiotic resistome in urban parks of Victoria, Australia.

摘要: The effluents from wastewater treatment plants have been recognized as a significant environmental reservoir of antibiotics and antibiotic resistance genes (ARGs). Reclaimed water irrigation (RWI) is increasingly used practical solution for combating scarcity in arid semiarid regions, however, impacts RWI on the patterns ARGs soil bacterial community remain unclear. Here, we high-throughput quantitative PCR terminal restriction fragment length polymorphism techniques to compare diversity, abundance composition broad-spectrum total bacteria 12 urban parks with without Victoria, Australia. A 40 unique were detected across all park soils, conferring β-lactam being most prevalent ARG type. numbers fold changes significantly increased by RWI, marked shifts also observed compared those RWI. paralleled effect structure co-occurrence pattern types. There positive correlations between integrase intI1 gene two (KPC IMP-2 groups), but no abundances transposase tnpA found, indicating that did not improve potential horizontal transfer ARGs. Taken together, our findings suggested reclaimed could influence abundance, compositions wide variety clinical relevance.Irrigation treated diversity various genes, enhance their transfer.