Method of whole genome amplification with reduced artifact production

摘要: Disclosed are compositions and methods for amplification of nucleic acid sequences interest with greater efficiency fidelity. The disclosed method relates to isothermal techniques, such as Multiple Displacement Amplification (MDA), where the generation DNA artifacts is decreased or eliminated. Generally, this can be accomplished by carrying out reaction at elevated temperature. In particularly useful embodiments method, sugars and/or other additives used stabilized polymerase high It has been discovered that molecular weight artifacts, in an procedure, substantially reduced eliminated while still allowing desired input a higher temperature and, optionally, presence one more additives. also reactions produce products quality, low bias, if performed