The Trypanosoma cruzi RNA-binding protein RBP42 is expressed in the cytoplasm throughout the life cycle of the parasite.

摘要: Trypanosoma cruzi, the protozoan parasite that causes Chagas disease in humans, has a complex life cycle promotes survival disparate environments. In each environment, must fine-tune its metabolic pathways to divide and multiply. absence of recognizable transcriptional gene regulation, it is apparent protein levels are determined by post-transcriptional mechanisms. Post-transcriptional control influenced RNA-binding proteins target mRNAs cell’s cytoplasm. To initiate study activities T. we studied this organism’s ortholog RBP42, trypanosomal protein. RBP42 was originally detected brucei shown subset encode governing central carbon metabolism. cruzi structurally resembles sharing an NTF2 domain at amino terminus single (specifically, RNA recognition motif, or RRM), carboxy terminus. A phylogenetic analysis reveals RRM distinguishing features all orthologs within broad kinetoplastid grouping. expressed stages as immunoblot immunofluorescence microscopy. case, localized cytoplasm, indicating role for cycle. We speculate influences dynamic responsible infection transmission.