Mass Spectrometry Investigation of Glycosylation Aberration via De-N-Glycopeptide Analysis

摘要: Proteomics research on glycan alterations has received great attention owing to their implications in disease initiation and progression. Determination of the glycoprotein expression remains one most challenging tasks as residues a given exist complex branched structures differ linkage. In view vital role changes cellular processes progression, there been an increased interest developing methodologies for detection these changes. A subset proteomics methods are discussed here that demonstrate utility glycan-free de-N-glycopeptide analysis screening glycoproteome well discovery glycopeptide/glycoprotein biomarkers.