Robustness of force and stress inference in an epithelial tissue

摘要: During morphogenesis, the shape of a tissue emerges from collective cellular behaviors, which are in part regulated by mechanical and biochemical interactions between cells. Quantification force stress is therefore necessary to analyze mechanisms controlling morphogenesis. Recently, measurement method based on inference cell shapes connectivity has been developed. It non-invasive, can provide space-time maps within an epithelial tissue, up prefactors. We previously performed comparative study three force-inference methods, differ their approach treating indefiniteness inverse problem forces. In present study, further validate compare we tested robustness measuring temporal fluctuation estimated Quantitative data cell-level dynamics developing suggests that variation forces will remain small short period time (~minutes). Here, showed cell-junction tensions global inferred Bayesian varied less with than those estimates only tension. contrast, amplitude fluctuations pressures differs different methods. Altogether, strengthens validity method.