Genetic detection of diarrheagenic Escherichia coli isolated from children with sporadic diarrhea.

摘要: Escherichia coli strains are among the major bacterial causes of diarrheal illness. At least 5 categories diarrheagenic E. (DEC) recognized, namely enterotoxigenic (ETEC), enteropathogenic (EPEC), enteroinvasive (EIEC), enteroaggregative (EAEC), and enterohemorrhagic (EHEC). Due to need for costly labor-intensive diagnostic procedures, identification DEC is difficult at standard laboratories. Therefore, epidemiology infections remains obscure in Taiwan. Recently, polymerase chain reaction (PCR) or dot blot has been used genetic detection DEC. In this study, we analyzed 150 isolates from stools children under years old. The PCR tests detected ETEC (3.3%), 6 EPEC (4%), 4 EIEC (2.7%), 13 EAEC (8.7%) isolates. No EHEC was detected. Dot sequence analysis were confirm results PCR. cellular fatty acid (CFA) profiles also analyzed. Comparison CFA composition revealed minor variation percentage each ETEC, EPEC, EAEC, but did not provide enough evidence differentiating between by alone.