Effect of Epidermal Growth Factor on Glioma Cell Growth, Migration, and Invasion in Vitro

摘要: Effects of epidermal growth factor (EGF) and an antibody (Ab-528) reactive against the binding site for EGF on human receptors were studied multicellular tumor spheroids obtained from three glioma cell lines with high (D-37 MG), medium (D-247 low (D-263 MG) levels receptor expression. The D-247 MG D-263 grew slowly or not at all in absence EGF, while presence they stimulated. Tumor migration, as measured by spread cells a plastic substratum, was increased addition lines. Stimulation migration could be blocked subsequent Ab-528 to concentration 50 micrograms/ml. Invasiveness into fetal rat brain aggregates related expression; two expression D-37 invasive, line noninvasive, assessed vitro coculture assay. In line, morphometry revealed EGF-enhanced invasiveness cells. EGF-treated cocultures reduced invasion both Antibody alone did affect but inhibit invasiveness. present study suggests that, tumors number normal-sized Mr 170,000 receptors, EGF-like ligand such transforming factor-alpha may selectively facilitate expansive invasion. This effect part retarded specific antibodies receptor.