Iron(III)-Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry for the Purification and Characterization of Synthetic Phosphopeptides

作者: Shihua Li , Chhabil Dass

DOI: 10.1006/ABIO.1999.4060

关键词: Sample preparation in mass spectrometryHydrophilic interaction chromatographyIon chromatographyChemistryProtein mass spectrometryDisplacement chromatographyLiquid chromatography–mass spectrometryHigh-performance liquid chromatographyColumn chromatographyChromatographyBiophysicsCell biologyBiochemistryMolecular biology

摘要: … A method based upon immobilized metal ion affinity … acetate–agarose gel. The method was applied for purification … The UV and total ion current chromatograms demonstrated …

参考文章(15)
John R. Yates, Protein and peptide analysis by mass spectrometry Analytica Chimica Acta. ,vol. 343, pp. 296- ,(1997) , 10.1016/S0003-2670(97)89600-6
R. C. Sheppard, E. Atherton, Solid phase peptide synthesis : a practical approach IRL Press. ,(1989)
Grażyna Muszyńska, Grażyna Dobrowolska, Anders Medin, Anders Medin, Pia Ekman, Jerker O. Porath, Model studies on iron(III) ion affinity chromatography. II. Interaction of immobilized iron(III) ions with phosphorylated amino acids, peptides and proteins. Journal of Chromatography A. ,vol. 604, pp. 19- 28 ,(1992) , 10.1016/0021-9673(92)85524-W
Frank A. Settle, Handbook of instrumental techniques for analytical chemistry Prentice Hall PTR. ,(1997)
Gregory A. Grant, Synthetic Peptides: A User's Guide ,(2002)
Grazyna Muszynska, Lennart Andersson, Jerker Porath, Selective adsorption of phosphoproteins on gel-immobilized ferric chelate. Biochemistry. ,vol. 25, pp. 6850- 6853 ,(1986) , 10.1021/BI00370A018
Lennart Andersson, Jerker Porath, Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography. Analytical Biochemistry. ,vol. 154, pp. 250- 254 ,(1986) , 10.1016/0003-2697(86)90523-3
Leonard D. Holmes, Martin R. Schiller, Immobilized Iron(III) Metal Affinity Chromatography for the Separation of Phosphorylated Macromolecules: Ligands and Applications Journal of Liquid Chromatography & Related Technologies. ,vol. 20, pp. 123- 142 ,(1997) , 10.1080/10826079708010641
Grigory Chaga, Lennart Andersson, Jerker Porath, Purification and determination of the binding site of lactate dehydrogenase from chicken breast muscle on immobilized ferric ions Journal of Chromatography A. ,vol. 627, pp. 163- 172 ,(1992) , 10.1016/0021-9673(92)87196-F