Regulation of flagellar length in Chlamydomonas

摘要: The length of eukaryotic cilia and flagella depends on the cell cycle-regulated assembly disassembly at least 9 doublet 2 central microtubules, their associated proteins, surrounding membrane. In light-synchronized Chlamydomonas cells, assembled to 10-14 microm in near beginning light period they disassembled prior division, during dark period. Flagella pf18 mutants grew 10-12 but shortened by 50% or more end Flagellar was cell-cycle regulated: when were amputated various times period, new regenerated lengths control cells that time cycle. later cycle deflagellated, shorter flagella. shortening not affected, either wild-type (wt) inhibitors protein synthesis microtubule assembly, so flagellar cannot depend turnover. Shortening attenuated Li+, which stimulated growth wt red light, kinase inhibitors, Ca2+ channel blockers La3+ Cd2+. increased cAMP, Na+, K+, EGTA. Ca2+-CAM did affect fa1 cells. We propose is regulated a signal transduction pathway sensitive levels light.