Selective excretion of alkaline xylanase by escherichia coli carrying PCX311
作者： Hiroshi HONDA , Toshiaki KUDO , Koki HORIKOSHI
关键词: Escherichia coli 、 Alkaline phosphatase 、 Xylanase 、 Molecular cloning 、 Biochemistry 、 Molecular biology 、 Enterobacteriaceae 、 Plasmid 、 Bacillaceae 、 Bacterial outer membrane 、 Chemistry
摘要: Plasmid pCX311, which we constructed, has two HindlU DNA fragments (2.6 kbp and 2.0 kbp) of alkalophilic Bacillus sp. strain C-125 in the site pBR322.These were essential not only for xylanase production but also excretion periplasmic proteins. The cloned 4.6 fragment encodes some components that made outer membrane E. coli permeable. Some proteins such as ²-lactamase excreted, alkaline phosphatase was excreted into culture broth.
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