Glial fibrillary acidic protein in the retina of the developing albino rat: An immunoperoxidase study of paraffin-embedded tissue
作者： Richard G. Dixon , Lawrence F. Eng
关键词: Paraformaldehyde 、 Optic nerve 、 Stain 、 Fixation (histology) 、 Retina 、 Immunoperoxidase 、 Glutaraldehyde 、 Pathology 、 Glial fibrillary acidic protein 、 Biology 、 General Neuroscience
摘要: The unlabeled peroxidase-anti-peroxidase method was used to stain glial fibrillary acidic (GFA) protein in the optic nerve of developing albino rat. Optic nerves from animals ranging age day birth adulthood were embedded paraffin following fixation with various agents for times 30 minutes 48 hours. GFA activity demonstrable at large astrocytic processes alcohols or Perfix short intervals, but not 4% 2% buffered paraformaldehyde solutions. With increasing age, could be demonstrated using higher aldehyde concentrations, longer times, and embedding schedules. At all ages greater treatment nonaldehyde fixatives rather than those containing formaldehyde glutaraldehyde. majority protein-containing confined planes which perpendicular axons nerve. tangential longitudinal became more numerous until, mature nerve, best characterized as being multidirectional.
sci-hub.se 参考文章(70)
Lawrence F. Eng, John W. Bigbee, Immunohistochemistry of Nervous System-Specific Antigens Springer US. pp. 43- 98 ,(1978) , 10.1007/978-1-4615-8240-3_2
S.J. De Armond, L.F. Eng, L.J. Rubinstein, The Application of Glial Fibrillary Acidic (GFA) Protein Immunohistochemistry in Neurooncology. A Progress Report Pathology Research and Practice. ,vol. 168, pp. 374- 394 ,(1980) , 10.1016/S0344-0338(80)80273-1
J S Bromberg, M Schachner, Localization of nervous system antigens in retina by immunohistology. Investigative Ophthalmology & Visual Science. ,vol. 17, pp. 920- 924 ,(1978)
B. Daicker, Die adventitiellen und intervasculären Reticulinfasern der menschlichen Netzhaut Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie. ,vol. 181, pp. 179- 191 ,(1971) , 10.1007/BF02390249
L. F. Eng, Y-L. Lee, K. Williams, G. Fukayama, B. Gerstl, M. Kies, Clearance of myelin basic protein from blood of normal and EAE rabbits. Advances in Experimental Medicine and Biology. ,vol. 100, pp. 329- 346 ,(1978) , 10.1007/978-1-4684-2514-7_24
Sanford L Palay, Alan Peters, Henry de F Webster, The fine structure of the nervous system: The neurons and supporting cells ,(1976)
M. Kidd, Electron microscopy of the inner plexiform layer of the retina in the cat and the pigeon Journal of Anatomy. ,vol. 96, pp. 179- 187 ,(1962)
Albert Kölliker, Mikroskopische Anatomie; oder, Gewebelehre des Menschen Readex Microprint. ,(1969)
M.J.Alvarado, Hogan, J.A.Weddell, Histology of the human eye;: An atlas and textbook ,(1971)
Arnaldo Lasansky, Functional Implications of Structural Findings in Retinal Glial Cells Progress in Brain Research. ,vol. 15, pp. 48- 72 ,(1965) , 10.1016/S0079-6123(08)60939-5