Metabolomics reveals the effect of Xuefu Zhuyu Decoction on plasma metabolism in rats with acute traumatic brain injury

摘要: // Dandan Feng 1 , Zian Xia Jing Zhou Hongmei Lu 2 Chunhu Zhang Rong Fan Xingui Xiong Hanjin Cui Pingping Gan 3 Wei Huang Weijun Peng 4 He 5 Zhiming Wang Yang and Tao Tang Institute of Integrative Chinese Medicine, Xiangya Hospital, Central South University, Changsha 410008, P.R. China Research Center Modernization Traditional Medicines, College Chemistry Chemical Engineering, 410083, Department Oncology, Integrated Western The Second 410011, Hepatobiliary Surgery, Correspondence to: Wang, email: wangyang_xy87@csu.edu.cn Tang, falcontang@126.com Keywords: metabolomics, acute traumatic brain injury, traditional medicine, Xuefu Zhuyu Decoction, gas chromatography/mass spectrometry Received: February 21, 2017      Accepted: September 03, Published: October 16, 2017 ABSTRACT Decoction (XFZY), an important herbal formula, has been reported effective on injury (TBI) in rats. However, its cerebral protection mechanism not clarified at the metabolic level. This work aims to explore global characteristics XFZY rats during phase TBI days 3. A plasma metabolomics method based chromatography-mass coupled with univariate analysis multivariate statistical was performed three groups (Sham, Vehicle, XFZY). Then, a pathway using MetaboAnalyst 3.0 illustrate pathways therapeutic action TBI. treatment attenuates neurological dysfunction cortical lesion volume post-injury day 3, reverses metabolite abnormalities (glutamic acid, lactic 3-hydroxybutyric ribitol, etc.). These differential metabolites are mainly involved D-glutamine D-glutamate metabolism, alanine, aspartate glutamate inositol phosphate metabolism. Our study reveals potential biomarkers networks neuroprotection effects XFZY, shows this approach would be feasible way systematically