Mutations in U6 snRNA that alter splice site specificity: implications for the active site
作者： C. Lesser , C Guthrie
关键词: RNA splicing 、 Genetics 、 Splice site mutation 、 Biology 、 Active site 、 Small nuclear RNA 、 Base pair 、 splice 、 Binding site 、 Intron
摘要: What determines the precise sites of cleavage in two transesterification reactions messenger RNA (mRNA) splicing is a major unsolved question. Mutation invariant G (guanosine) at position 5 5' splice Saccharomyces cerevisiae introns activates nearby aberrant sites. A genetic approach was used to test hypothesis that base-pairing interaction between site and ACAGAG sequence U6 determinant choice. Mutations or intron (or both) were predicted stabilize suppressed increased normal cleavage. In addition, mutation mutations 3' dinucleotide. These data can fit model for spliceosomal active comprised set RNA-RNA interactions intron, U2 U6.
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S F Jamison, A Crow, M A Garcia-Blanco, The spliceosome assembly pathway in mammalian extracts. Molecular and Cellular Biology. ,vol. 12, pp. 4279- 4287 ,(1992) , 10.1128/MCB.12.10.4279
John L. Woolford, Nuclear pre-mRNA splicing in yeast. Yeast. ,vol. 5, pp. 439- 457 ,(1989) , 10.1002/YEA.320050604
Lynette A. Fouser, James D. Friesen, Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing Cell. ,vol. 45, pp. 81- 93 ,(1986) , 10.1016/0092-8674(86)90540-4
Andrew Newman, Christine Norman, Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage Cell. ,vol. 65, pp. 115- 123 ,(1991) , 10.1016/0092-8674(91)90413-S
D. Wassarman, J. Steitz, Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing. Science. ,vol. 257, pp. 1918- 1925 ,(1992) , 10.1126/SCIENCE.1411506
Hiten D. Madhani, Christine Guthrie, A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell. ,vol. 71, pp. 803- 817 ,(1992) , 10.1016/0092-8674(92)90556-R
M. Green, Biochemical Mechanisms Of Constitutive And Regulated Pre-Messenger RNA Splicing Annual Review of Cell and Developmental Biology. ,vol. 7, pp. 559- 599 ,(1991) , 10.1146/ANNUREV.CELLBIO.7.1.559
Alain Jacquier, Jose R. Rodriguez, Michael Rosbash, A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing Cell. ,vol. 43, pp. 423- 430 ,(1985) , 10.1016/0092-8674(85)90172-2
C Guthrie, Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein Science. ,vol. 253, pp. 157- 163 ,(1991) , 10.1126/SCIENCE.1853200
A.J. Newman, C. Norman, U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites Cell. ,vol. 68, pp. 743- 754 ,(1992) , 10.1016/0092-8674(92)90149-7