Web based algorithms EvoPrinter and cis-Decoder reveal functional sequences in enhancers and complex networks of transcription factor interactions required for gene regulation

摘要: Arabidopsis root development provides a remarkably tractable system to delineate tissue-specific, developmental gene regulatory networks and study their functionality in complex multicellular model over time. Tightly controlled expression within tissues is hallmark of accomplished by transcription factors (TFs) microRNAs (miRNAs). We present an automated, enhanced yeast one hybrid (eY1H) assay using tissue-specific TF resource comprehensively map the stele. These are robust highly combinatorial nature. Using these methods computational modeling, we have additionally modeled network that regulates distinct transcriptional events Distinct modules were identified temporally drive genes involved xylem specification subsequent synthesis secondary cell wall metabolites associated with differentiation.