Isolation of a low molecular mass vasoactive intestinal peptide binding protein.
作者： C.H. Brugger , D. Stallwood , S. Paul
DOI: 10.1016/S0021-9258(18)55278-9
关键词: Vasoactive intestinal peptide 、 Biology 、 High-performance liquid chromatography 、 Molecular mass 、 Gel electrophoresis 、 Size-exclusion chromatography 、 Protein purification 、 Binding protein 、 Biochemistry 、 Chromatography 、 Affinity chromatography
摘要: A vasoactive intestinal peptide (VIP) binding protein was purified in active form by detergent solubilization of lung membranes, gel filtration, VIP-Sepharose affinity chromatography, reverse phase high performance liquid and anion exchange chromatography. The mass this estimated at 18 kDa sodium dodecyl sulfate-polyacrylamide electrophoresis 17 filtration. VIP inhibited Mg2+, covalent cross-linking [Tyr10-125I]VIP to the produced two radioactive bands 22 26 identified electrophoresis, exhibited saturable related neuropeptide, rat growth hormone releasing factor.
sci-hub.st 参考文章(27)
A Couvineau, T Voisin, L Guijarro, M Laburthe, Purification of vasoactive intestinal peptide receptor from porcine liver by a newly designed one-step affinity chromatography. Journal of Biological Chemistry. ,vol. 265, pp. 13386- 13390 ,(1990) , 10.1016/S0021-9258(19)38310-3
S Paul, S I Said, Characterization of receptors for vasoactive intestinal peptide solubilized from the lung. Journal of Biological Chemistry. ,vol. 262, pp. 158- 162 ,(1987) , 10.1016/S0021-9258(19)75903-1
J R Zysk, M J Cronin, J M Anderson, M O Thorner, Cross-linking of a growth hormone releasing factor-binding protein in anterior pituitary cells. Journal of Biological Chemistry. ,vol. 261, pp. 16781- 16784 ,(1986) , 10.1016/S0021-9258(19)75955-9
S Patthi, M Akong, G Veliçelebi, Hydrodynamic characterization of vasoactive intestinal peptide receptors extracted from rat lung membranes in Triton X-100 and n-octyl-beta-D-glucopyranoside. Journal of Biological Chemistry. ,vol. 262, pp. 15740- 15745 ,(1987) , 10.1016/S0021-9258(18)47790-3
A el Battari, J M Martin, J Luis, O Pouzol, J Secchi, J Marvaldi, J Pichon, Solubilization of the active vasoactive intestinal peptide receptor from human colonic adenocarcinoma cells. Journal of Biological Chemistry. ,vol. 263, pp. 17685- 17689 ,(1988) , 10.1016/S0021-9258(19)77891-0
R K Malhotra, T D Wakade, A R Wakade, Vasoactive intestinal polypeptide and muscarine mobilize intracellular Ca2+ through breakdown of phosphoinositides to induce catecholamine secretion. Role of IP3 in exocytosis. Journal of Biological Chemistry. ,vol. 263, pp. 2123- 2126 ,(1988) , 10.1016/S0021-9258(18)69179-3
A Couvineau, B Amiranoff, M Laburthe, Solubilization of the liver vasoactive intestinal peptide receptor. Hydrodynamic characterization and evidence for an association with a functional GTP regulatory protein. Journal of Biological Chemistry. ,vol. 261, pp. 14482- 14489 ,(1986) , 10.1016/S0021-9258(18)66895-4
Deanna J. Volle, Sudhir Paul, Sun Mei, Affinity chromatography of catalytic autoantibody to vasoactive intestinal peptide. Journal of Immunology. ,vol. 145, pp. 1196- 1199 ,(1990)
Pierre Chatelain, Patrick Robberecht, Magali Waelbroeck, Philippe De Neef, Jean-Claude Camus, Auh Nguyen Huu, Joseph Roba, Jean Christophe, Topographical distribution of the secretin- and VIP-stimulated adenylate cyclase system in the heart of five animal species. Pflügers Archiv: European Journal of Physiology. ,vol. 397, pp. 100- 105 ,(1983) , 10.1007/BF00582046
M Schramm, Z Selinger, Message transmission: receptor controlled adenylate cyclase system Science. ,vol. 225, pp. 1350- 1356 ,(1984) , 10.1126/SCIENCE.6147897