Chemotaxis, viability, and labeling stability of leukocytes labeled with (99m)Tc-exametazime stabilized with methylene blue.
作者： Manuel Roca , Fernando Iglesias , Víctor García , Félix Armero , Ma Carmen Díaz
DOI:
关键词: Chemistry 、 Methylene blue 、 In vitro 、 Viability assay 、 Eosin Y 、 Staining 、 Chemotaxis 、 Technetium Tc 99m Exametazime 、 Biochemistry 、 Chromatography 、 Technetium
摘要: The in vitro viability, chemotaxis, and labeling stability of leukocytes labeled using 99mTc-exametazime stabilized with methylene blue were evaluated compared those obtained nonstabilized 99mTc-exametazime. Methods: Two identical leukocyte populations, from 30 healthy donors, simultaneously freshly prepared 2-h-old following quality control techniques performed on each sample: eosin Y staining, chemotaxis radioassay, at 2 h after labeling. Results: Eosin staining showed a cell viability least 98% all samples, without significant statistical difference between the populations. Chemotactic indices prepared, unstabilized statistically greater than (z = 2.41; P
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