作者: R. Ordinola-Zapata , C. M. Bramante , B. Cavenago , M. S. Z. Graeff , I. Gomes de Moraes
DOI: 10.1111/J.1365-2591.2011.01959.X
关键词: Dentistry 、 Food science 、 Chlorhexidine 、 Bovine dentine 、 Antimicrobial effect 、 Sodium hypochlorite 、 Materials science 、 Dentin 、 Citric acid 、 Biofilm 、 Distilled water
摘要: Ordinola-Zapata R, Bramante CM, Cavenago B, Graeff MSZ, Gomes de Moraes I, Marciano M, Duarte MAH. Antimicrobial effect of endodontic solutions used as final irrigants on a dentine biofilm model. International Endodontic Journal, 45, 162–168, 2012. Abstract Aim To evaluate the residual biovolume live bacterial cells, mean thickness and substratum coverage found in mixed biofilms treated with different irrigant solutions. Methodology Twenty-five bovine specimens were infected intraorally using removable orthodontic device. Five samples for each solution: 2% chlorhexidine, 1% sodium hypochlorite (NaOCl), 10% citric acid, 17% EDTA distilled water. The 5 min. stained Live/Dead technique evaluated confocal microscope. Differences amount total (μm3), number surviving cells (μm) (%) determined nonparametric statistical tests (P < 0.05). Results Similar values total, subpopulations water-treated (P > 0.05). lower studied parameters NaOCl-treated (P 0.05). Conclusions One per cent was only that had significant viability architecture.