Antimicrobial effect of endodontic solutions used as final irrigants on a dentine biofilm model.

作者: R. Ordinola-Zapata , C. M. Bramante , B. Cavenago , M. S. Z. Graeff , I. Gomes de Moraes

DOI: 10.1111/J.1365-2591.2011.01959.X

关键词: DentistryFood scienceChlorhexidineBovine dentineAntimicrobial effectSodium hypochloriteMaterials scienceDentinCitric acidBiofilmDistilled water

摘要: Ordinola-Zapata R, Bramante CM, Cavenago B, Graeff MSZ, Gomes de Moraes I, Marciano M, Duarte MAH. Antimicrobial effect of endodontic solutions used as final irrigants on a dentine biofilm model. International Endodontic Journal, 45, 162–168, 2012. Abstract Aim  To evaluate the residual biovolume live bacterial cells, mean thickness and substratum coverage found in mixed biofilms treated with different irrigant solutions. Methodology  Twenty-five bovine specimens were infected intraorally using removable orthodontic device. Five samples for each solution: 2% chlorhexidine, 1% sodium hypochlorite (NaOCl), 10% citric acid, 17% EDTA distilled water. The 5 min. stained Live/Dead technique evaluated confocal microscope. Differences amount total (μm3), number surviving cells (μm) (%) determined nonparametric statistical tests (P < 0.05). Results  Similar values total, subpopulations water-treated (P > 0.05). lower studied parameters NaOCl-treated (P   0.05). Conclusions  One per cent was only that had significant viability architecture.

参考文章(30)
J R Lawrence, D R Korber, B D Hoyle, J W Costerton, D E Caldwell, Optical sectioning of microbial biofilms. Journal of Bacteriology. ,vol. 173, pp. 6558- 6567 ,(1991) , 10.1128/JB.173.20.6558-6567.1991
N.B. Arweiler, E. Hellwig, A. Sculean, N. Hein, T.M. Auschill, Individual Vitality Pattern of in situ Dental Biofilms at Different Locations in the Oral Cavity Caries Research. ,vol. 38, pp. 442- 447 ,(2004) , 10.1159/000079625
Luis E. Chávez de Paz, Gunnar Bergenholtz, Gunnel Svensäter, The effects of antimicrobials on endodontic biofilm bacteria. Journal of Endodontics. ,vol. 36, pp. 70- 77 ,(2010) , 10.1016/J.JOEN.2009.09.017
Graeme Bryce, Darren O'Donnell, Derren Ready, Yuan-ling Ng, Jonathan Pratten, Kishor Gulabivala, Contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms. Journal of Endodontics. ,vol. 35, pp. 1243- 1248 ,(2009) , 10.1016/J.JOEN.2009.05.034
José F. Siqueira, Marcelo M.D. Batista, Ricardo C. Fraga, Milton de Uzeda, Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria. Journal of Endodontics. ,vol. 24, pp. 414- 416 ,(1998) , 10.1016/S0099-2399(98)80023-X
Thomas R. Dunavant, John D. Regan, Gerald N. Glickman, Eric S. Solomon, Allen L. Honeyman, Comparative Evaluation of Endodontic Irrigants against Enterococcus faecalis Biofilms Journal of Endodontics. ,vol. 32, pp. 527- 531 ,(2006) , 10.1016/J.JOEN.2005.09.001
Anca Virtej, Colin R. MacKenzie, Wolfgang H.-M. Raab, Klaus Pfeffer, Claudia R. Barthel, Determination of the performance of various root canal disinfection methods after in situ carriage. Journal of Endodontics. ,vol. 33, pp. 926- 929 ,(2007) , 10.1016/J.JOEN.2006.11.025
María Teresa Arias-Moliz, Carmen María Ferrer-Luque, Miguel Espigares-García, Pilar Baca, Enterococcus faecalis biofilms eradication by root canal irrigants Journal of Endodontics. ,vol. 35, pp. 711- 714 ,(2009) , 10.1016/J.JOEN.2009.01.018