Exploiting the difference between intrinsic and extrinsic kinases: implications for regulation of signaling by immunoreceptors.

摘要: When receptors must interact with an extrinsic kinase to initiate signaling, the can play a regulatory role that is not available intrinsic receptor kinases. Whether control exercised at this level depends critically on amount of and potential for redistribution during signaling. This study demonstrates high affinity IgE (Fc epsilonRI) rat basophilic leukemia cells regulated by its initiating kinase. We present mathematical model allows reversible recruitment kinases phosphorylated immunoreceptor tyrosine-based activation motifs. By comparing predictions experimental time courses phosphorylation, we infer Lyn limiting, occurs after are aggregated, makes relationship between tyrosine phosphorylation aggregation nonlinear.