Light and electron microscopic study on the endocrine cells of the pancreas in a marine teleost, Fugu rubripes rubripes

摘要: The pancreatic endocrine tissue of Fugu rubripes consists numerous round principal islets (Brockmann bodies) various sizes scattered around the gall-bladder. cells are divided into A-, B-, D-, and Ff-cells. Each cell type was identified by comparing thick thin sections in both light electron microscopy. Aldehyde-fuchsin positive B-cells contain secretory granules (average diameter 300 nm) each which has a compact core moderate density; narrow space exists between this limiting membrane. Grimelius' silver A 360 with hexagonal or tetragonal crystalline 170 high grains preferentially appear membrane core. α-granule often contains an appendix-like structure variable shape. D blackened impregnation method Hellman Hellerstrom (1960) have 320 filled flocculent material low density. fourth (Ff-cell) clear cytoplasm after differential staining for By microscopy, elongated fusiform (520 nm average length × 230 width) filaments arranged parallel longitudinal axis. Figures suggesting granule formation sacs Golgi apparatus were obtained all islet types. Equivalents emiocytotic release encountered Ff cells.