The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing
作者： Janet G Yang , Tina Shahian Madrid , Elena Sevastopoulos , Geeta J Narlikar
DOI: 10.1038/NSMB1170
关键词: Chromatin 、 Förster resonance energy transfer 、 Molecular biology 、 Linker DNA 、 Nucleosome 、 Chromatin remodeling 、 Biophysics 、 DNA 、 Solenoid (DNA) 、 Biology 、 Linker
摘要: Arrays of regularly spaced nucleosomes directly correlate with closed chromatin structures at silenced loci. The ATP-dependent chromatin-assembly factor (ACF) generates such arrays in vitro and is required for transcriptional silencing vivo. A key unresolved question how ACF 'measures' equal spacing between nucleosomes. We show that senses flanking DNA length transduces information an manner to regulate the rate nucleosome movement. Using fluorescence resonance energy transfer follow movement, we find can rapidly sample on either side a moves longer across faster than shorter DNA. This dynamic equilibrium which having accumulate. Our results indicate characteristic 50- 60-base-pair internucleosomal silent by kinetically discriminating against linker DNAs.
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sci-hub.se 参考文章(44)
Patrick D Varga-Weisz, Matthias Wilm, Edgar Bonte, Katia Dumas, Matthias Mann, Peter B Becker, None, Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II Nature. ,vol. 388, pp. 598- 602 ,(1997) , 10.1038/41587
Karolin Luger, Thomas J. Rechsteiner, Timothy J. Richmond, Preparation of nucleosome core particle from recombinant histones. Methods in Enzymology. ,vol. 304, pp. 3- 19 ,(1999) , 10.1016/S0076-6879(99)04003-3
Gaku Mizuguchi, Toshio Tsukiyama, Jan Wisniewski, Carl Wu, Role of Nucleosome Remodeling Factor NURF in Transcriptional Activation of Chromatin Molecular Cell. ,vol. 1, pp. 141- 150 ,(1997) , 10.1016/S1097-2765(00)80015-5
Martin Zofall, Jim Persinger, Stefan R Kassabov, Blaine Bartholomew, Chromatin remodeling by ISW2 and SWI/SNF requires DNA translocation inside the nucleosome Nature Structural & Molecular Biology. ,vol. 13, pp. 339- 346 ,(2006) , 10.1038/NSMB1071
Ali Hamiche, Raphael Sandaltzopoulos, David A Gdula, Carl Wu, ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF. Cell. ,vol. 97, pp. 833- 842 ,(1999) , 10.1016/S0092-8674(00)80796-5
Andrew Flaus, Tom Owen-Hughes, Dynamic properties of nucleosomes during thermal and ATP-driven mobilization. Molecular and Cellular Biology. ,vol. 23, pp. 7767- 7779 ,(2003) , 10.1128/MCB.23.21.7767-7779.2003
Jeff D. Aalfs, Geeta J. Narlikar, Robert E. Kingston, Functional Differences between the Human ATP-dependent Nucleosome Remodeling Proteins BRG1 and SNF2H Journal of Biological Chemistry. ,vol. 276, pp. 34270- 34278 ,(2001) , 10.1074/JBC.M104163200
P.T Lowary, J Widom, New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning. Journal of Molecular Biology. ,vol. 276, pp. 19- 42 ,(1998) , 10.1006/JMBI.1997.1494
Iestyn Whitehouse, Toshio Tsukiyama, Antagonistic forces that position nucleosomes in vivo Nature Structural & Molecular Biology. ,vol. 13, pp. 633- 640 ,(2006) , 10.1038/NSMB1111
Hua-Ying Fan, Xi He, Robert E. Kingston, Geeta J. Narlikar, Distinct Strategies to Make Nucleosomal DNA Accessible Molecular Cell. ,vol. 11, pp. 1311- 1322 ,(2003) , 10.1016/S1097-2765(03)00192-8