Antimicrobial activity of southern African medicinal plants with dermatological relevance: From an ethnopharmacological screening approach, to combination studies and the isolation of a bioactive compound

摘要: Abstract Ethnopharmacological relevance Ethnobotanical reports on more than 100 southern African medicinal plants with dermatological have been highlighted, yet there is still limited scientific data to support claims for their antimicrobial effectiveness against skin pathogens. Guided by ethnobotanical data, this paper explores the efficacies of used treat ailments. Aim study To investigate properties dermatologically relevant The also aimed at providing a rationale traditional use plant combinations diseases and isolation bio-active compound from most active species, Aristea ecklonii (Iridaceae). Materials methods Organic aqueous extracts (132) were prepared 47 species screened pathogens using micro-titre plate dilution method. Four different investigated interactive sum fractional inhibitory concentration (ƩFIC) calculated. Isobolograms further Pentanisia prunelloides combined Elephantorrhiza elephantina varied ratios. A bioactivity-guided fractionation process was adopted fractionate organic leaf extract ecklonii. Results Plants demonstrating notable broad-spectrum activities (MIC values ≤1.00 mg/ml) tested included ecklonii, Chenopodium ambrosioides, Diospyros mespiliformis, elephantina, Eucalyptus camaldulensis, Gunnera perpensa, Harpephyllum caffrum, Hypericum perforatum, Melianthus comosus, Terminalia sericea Warburgia salutaris. reportedly acne vulgaris, demonstrated noteworthy activity value 0.05 mg/ml) Propionibacterium acnes. Similarly, mespiliformis reported its ringworm, displayed Trichophyton mentagrophytes 0.10 mg/ml) Microsporum canis 0.50 mg/ml). root (1:1) synergistic interactions (ƩFIC 0.31–0.38) Staphylococcus aureus, gentamycin–methicillin resistant epidermidis Candida albicans. Fractionation resulted in known compound, plumbagin, displaying range between 2.00 μg/ml 16.00 μg/ml). Conclusion Most pathogen specific effects few exhibiting activities. Positive noted such as vulgaris ringworm infections, respectively, give some validation traditiona l uses. Synergistic validate an enhanced effect when combination. Noteworthy 16.00 μg/ml) observed plumbagin isolated