Induction of apoptosis in glioma cell lines by TRAIL/Apo-2l.
作者： Mian Wu , Asha Das , Yan Tan , Cong-ju Zhu , Taian Cui
DOI: 10.1002/1097-4547(20000815)61:4<464::AID-JNR14>3.0.CO;2-G
关键词: Tumor necrosis factor alpha 、 Molecular biology 、 Programmed cell death 、 Glioma 、 Cell biology 、 Flow cytometry 、 Cytokine 、 Cycloheximide 、 Apoptosis 、 Cell 、 Biology
摘要: TRAIL/Apo-2L, a novel cytokine, is member of the tumor necrosis factor (TNF) family and serves as an extracellular signal triggering apoptosis. TRAIL/Apo-2L capable killing various transformed cells but not unstimulated primary T cells. In this study, five human glioma (U87, U118, U178, U563, A172) were examined for their susceptibility to apoptotic effects TRAIL/Apo-2L. cDNA was isolated by RT-PCR, recombinant protein purified pMAL-c2 system (New England Biolabs, Beverly, MA). Exposure A172 bacterially expressed soluble fusion at concentration 1 μg/ml resulted in significant cell death over 24-h period. Three experiments performed suggest that through induction apoptosis target addition, expression different found be variable, TRAIL/Apo-2L–induced type-dependent manner. Some correlation between level one its cognate receptors, DR4, observed. cycloheximide worked synergistically with induce J. Neurosci. Res. 61:464–470, 2000. © 2000 Wiley-Liss, Inc.
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