Effects of Salts on the Conformation and Catalytic Properties of D-Amino Acid Aminotransferase
作者： Hyeon-Su Ro
DOI: 10.5483/BMBREP.2002.35.3.306
关键词: Circular dichroism 、 Medicinal chemistry 、 Size-exclusion chromatography 、 D-Alanine Transaminase 、 Biochemistry 、 Chemistry 、 Salt (chemistry) 、 Catalysis 、 Sodium 、 Spectroscopy 、 Protein structure
摘要: Received 29 December 2001, Accepted 27 February 2002The effects of salts on the biochemical properties D-amino acid aminotransferase from Bacillus sp. YM-1 havebeen studied to elucidate both inhibitory saltson activity and protective thesubstrate-induced inactivation. The results UV-visible spectroscopy studies reaction enzymewith D-serine revealed that salt significantly reduced therate formation quinonoid intermediate itsaccumulation. kinetic thereaction with α-[
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