Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

摘要: With the recent development of retinal prostheses, it is important to develop reliable techniques for assessing safety these devices in preclinical studies. However, standard fixation, preparation, and automated histology procedures are not ideal. Here we describe new evaluating health retina directly adjacent an implant. Retinal prostheses feature electrode arrays contact with eye tissue. Previous methods have been able spatially localize ocular tissue individual electrodes within array. In addition, histological processing often results gross artifactual detachment layers when implanted eyes. Consequently, has difficult assess localized damage, if present, caused by implantation stimulation Therefore, developed a method identifying localizing using (color-coded) dye marking scheme, modified fixation technique minimize detachment. This also rendered sclera translucent, enabling localization specific parts Finally, used matched control increase power histopathological assessments. summary, this enables efficient discrimination assessment cytoarchitecture eye.