Large volume sample stacking for rapid and sensitive determination of antidiabetic drug metformin in human urine and serum by capillary electrophoresis with contactless conductivity detection.

摘要: Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of is performed on a separation capillary an effective length 14 cm, using maximum voltage 30 kV small injection 50-fold diluted into capillary. Under these conditions, migration time 35s LOD 0.3 μM. Large-volume sample stacking was used to determine low levels serum. serum deproteinized acetonitrile 10 times greater compared injected amount urine. This enabled reduction 0.03 μM equalled 86 s. undesirable solvent from zone forced out ensure rapidity good repeatability determination. RSD values are 0.1% 0.7% serum; peak areas 1.4% 2.6% technique tested performance routine analyses patients suffering type II diabetes mellitus.