Phosphate concentration regulates transcription of the Acinetobacter polyhydroxyalkanoic acid biosynthetic genes.
作者： M A Schembri , R C Bayly , J K Davies
DOI: 10.1128/JB.177.15.4501-4507.1995
关键词: Open reading frame 、 Gene 、 Biology 、 Peptide sequence 、 Biochemistry 、 Genetics 、 Regulon 、 Operon 、 Nucleic acid sequence 、 Locus (genetics) 、 Promoter
摘要: The polyhydroxyalkanoic acid (PHA) biosynthetic gene locus was cloned and characterized from an Acinetobacter sp. isolated activated sludge. Nucleotide sequence analysis identified three clustered genes, phaAAc (encoding a beta-ketothiolase), phaBAc acetoacetyl coenzyme A reductase), phaCAc PHA synthase). In addition, open reading frame (ORF1) with potential to encode 13-kDa protein within this locus. of the putative translational product ORF1 does not show significant similarity any sequences in database. plasmid containing pha conferred ability accumulate poly-beta-hydroxybutyrate on its Escherichia coli host. These genes appear lie operon transcribed by two promoters upstream phaBAc, apparent constitutive promoter, second promoter induced phosphate starvation under pho regulon control. as well number additional transcription start points were combination primer extension promoter-chloramphenicol acetyltransferase fusion studies carried out or E. transformants.
uq.edu.au
core.ac.uk参考文章(22)
O P Peoples, A J Sinskey, Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase. Journal of Biological Chemistry. ,vol. 264, pp. 15293- 15297 ,(1989) , 10.1016/S0021-9258(19)84824-X
Kozo Makino, Hideo Shinagawa, Mitsuko Amemura, Sigenobu Kimura, Atsuo Nakata, Akira Ishihama, Regulation of the phosphate regulon of Escherichia coli: Activation of pstS transcription by PhoB protein in vitro Journal of Molecular Biology. ,vol. 203, pp. 85- 95 ,(1988) , 10.1016/0022-2836(88)90093-9
M Kasahara, K Makino, M Amemura, A Nakata, H Shinagawa, Dual regulation of the ugp operon by phosphate and carbon starvation at two interspaced promoters. Journal of Bacteriology. ,vol. 173, pp. 549- 558 ,(1991) , 10.1128/JB.173.2.549-558.1991
U Pieper-Fürst, M H Madkour, F Mayer, A Steinbüchel, Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber. Journal of Bacteriology. ,vol. 176, pp. 4328- 4337 ,(1994) , 10.1128/JB.176.14.4328-4337.1994
Edith Brickman, Larry Soll, Jon Beckwith, Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. Journal of Bacteriology. ,vol. 116, pp. 582- 587 ,(1973) , 10.1128/JB.116.2.582-587.1973
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
A J Anderson, E A Dawes, Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiological Research. ,vol. 54, pp. 450- 472 ,(1990) , 10.1128/MR.54.4.450-472.1990
B. L. Wanner, Gene regulation by phosphate in enteric bacteria Journal of Cellular Biochemistry. ,vol. 51, pp. 47- 54 ,(1993) , 10.1002/JCB.240510110
O. P. Peoples, A. J. Sinskey, Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding beta-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB. Molecular Microbiology. ,vol. 3, pp. 349- 357 ,(1989) , 10.1111/J.1365-2958.1989.TB00180.X
Mark A. Schembri, Ronald C. Bayly, John K. Davies, Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located. Fems Microbiology Letters. ,vol. 118, pp. 145- 152 ,(1994) , 10.1111/J.1574-6968.1994.TB06817.X