Isolation and Characterization of a Light-Harvesting Chlorophyll a/b Protein Complex Associated with Photosystem I
作者： Eric Lam , William Oritz , Stephen Mayfield , Richard Malkin
DOI: 10.1104/PP.74.3.650
关键词: Chlorophyll a 、 Sephadex 、 Light-harvesting complexes of green plants 、 Photosystem II 、 Biochemistry 、 Fluorescence 、 Absorbance 、 Chemistry 、 Photosystem I 、 Gel electrophoresis 、 Plant science 、 Genetics 、 Physiology
摘要: A chlorophyll a/b protein complex has been isolated from a resolved native photosystem I by mildly dissociating sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The contains single polypeptide of molecular weight 20 kilodaltons, and ratio 3.5 to 4.0. visible absorbance spectrum the showed maximum at 667 nanometers in red region 77 K fluorescence emission 681 nanometers. Alternatively, treatment with lithium sulfate Triton, could be chromatography on Sephadex G-75. Immunological assays using antibodies P700-chlorophyll a-protein II light-harvesting show no cross-reaction between other two chlorophyll-containing complexes.
plantphysiol.org参考文章(17)
Nam-Hai Chua, [40] Electrophoretic analysis of chloroplast proteins Methods in Enzymology. ,vol. 69, pp. 434- 446 ,(1980) , 10.1016/S0076-6879(80)69042-9
Philip Haworth, Janet L. Watson, Charles J. Arntzen, The detection, isolation and characterization of a light-harvesting complex which is specifically associated with Photosystem I Biochimica et Biophysica Acta. ,vol. 724, pp. 151- 158 ,(1983) , 10.1016/0005-2728(83)90036-1
Joan Argyroudi-Akoyunoglou, Helen Thomou, Separation of thylakoid pigment—protein complexes by SDS—sucrose density gradient centrifugation FEBS Letters. ,vol. 135, pp. 177- 181 ,(1981) , 10.1016/0014-5793(81)80971-4
Francis-André Wollman, Pierre Bennoun, A new chlorophyll-protein complex related to Photosystem I in Chlamydomonas reinhardii Biochimica et Biophysica Acta. ,vol. 680, pp. 352- 360 ,(1982) , 10.1016/0005-2728(82)90149-9
Reto J. Strasser, Warren L. Butler, Energy transfer in the photochemical apparatus of flashed bean leaves Biochimica et Biophysica Acta. ,vol. 449, pp. 412- 419 ,(1976) , 10.1016/0005-2728(76)90152-3
Peter R. Dunkley, Jan M. Anderson, The light-harvesting chlorophyll ab-protein complex from barley thylakoid membranes. Polypeptide composition and characterization of an oligomer Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 545, pp. 175- 187 ,(1979) , 10.1016/0005-2728(79)90124-5
Tetsuo Hiyama, Bacon Ke, Difference spectra and extinction coefficients of P700 Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 267, pp. 160- 171 ,(1972) , 10.1016/0005-2728(72)90147-8
J. M. Anderson, A. Melis, Localization of different photosystems in separate regions of chloroplast membranes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 745- 749 ,(1983) , 10.1073/PNAS.80.3.745
Anastasios Melis, Jan M. Anderson, Structural and functional organization of the photosystems in spinach chloroplasts:Antenna size, relative electron transport capacity, and chlorophyll composition Biochimica et Biophysica Acta. ,vol. 724, pp. 473- 484 ,(1983) , 10.1016/0005-2728(83)90108-1
W. L. Butler, R. J. Strasser, Tripartite model for the photochemical apparatus of green plant photosynthesis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 3382- 3385 ,(1977) , 10.1073/PNAS.74.8.3382