Mechanism of Bacterial Translation Termination and Ribosome Recycling

摘要: The elucidation of the role release factor 3 (RF3) in translation termination and demonstration that GTP hydrolysis was needed for RF3 action became possible with development improved purified vitro systems protein synthesis. In absence RF3, inhibitory RF1 on ribosome recycling can be reduced by increasing ribosome-recycling (RRF) concentration, suggesting whatever RRF may be, it is incompatible presence ribosome. experiments system show from initiation via elongation synthesis depends strictly IF3. This implies EF-G together split into its subunits after this step overture to back posttermination state. fate mRNA highly relevant multicistronic mRNAs. data suggest when followed RRF, EF-G, IF3 30S particle remains attached mRNA, allowing latter diffuse long distances along subunit. GTP, accelerates RF2 enhancing their rates dissociation translation.