Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing

摘要: Human gut microbiota plays important roles in harvesting energy from the diet, stimulating proliferation of intestinal epithelium, developing immune system, and regulating fat storage host. Characterization microbiota, however, has been limited to western people is not sufficiently extensive fully describe microbial communities. In this study, we investigated overall composition its host specificity temporal stability 20 Koreans using 454-pyrosequencing with barcoded primers targeting V1 V3 region bacterial 16S rRNA gene. A total 303,402 high quality reads covered each sample 8,427 were analyzed on average. The results compared those individuals USA, China Japan. general, communities dominated by five previously identified phyla: Actinobacteria, Firmicutes, Bacteroidetes, Fusobacteria, Proteobacteria. UPGMA cluster analysis showed that species was host-specific stable over duration test period, but relative abundance member fluctuated. 43 core Korean comparison sequences individual, which 15 level phylotypes related previously-reported butyrate-producing bacteria. UniFrac revealed human differed between countries: Korea, Japan China, tended vary less individual Koreans, suggesting internal external characteristics country such as genetics diet styles.