作者: Maznah Ismail
DOI:
关键词: Sephadex 、 Fractionation 、 Chromatography 、 Acetone 、 Spirodela 、 Trypsin inhibitor 、 Methionine 、 Essential amino acid 、 Wolffia columbiana 、 Chemistry
摘要: The amount of protein was determined in supernatant and residual pulp three species duckweed, Spirodela Polyrrhiza, oligorrhiza, Wolffia columbiana and a mixed culture. Protein content the similar except S. oligorrhiza which had triple value. Maznah Ismail Precipitation studies with acid organic solvents (acetone, isobutanol isopropanol) showed that precipitated varied the solvent used. On average, only about 20% from supernatant. More by (pH 5.8) isopropanol (5% v/v). Amino analysis essential amino whole frond, compared favourably human cow's milk, egg FAO reference pattern, exception methionine. Fractionation soluble material using Sephacryl S-200 and Sephadex G-50 yielded six components seven components. first component was> 250,000 daltons size last two < 10,000. Further fractionation anion exchanger, DJ<.AE Sepharose CL-6B 12 components, W columlbiana, 9. net positive charge while rest negative change. Anti-nutritional factors were also duckweed (Spirodela polyrrhiza Lemna perpusilla Torrey) their concentrates. found to be low; higher values than for trypsin inhibitor. However variance no significant difference (p ~0.05) levels these anti nutritional between species.