Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro.

摘要: A rapid in vitro cytolytic effect of some myotoxic phospholipases A2 (PLA2s) isolated from the venoms Viperidae snakes has been previously described. This study was undertaken to investigate if activity is a common property proteins this group. Murine endothelial cells (tEnd) and skeletal muscle myotubes (C2C12) were utilized as targets. The release lactic dehydrogenase quantified measure cell damage, 3 h after exposure different PLA2s, including representatives genera Bothrops, Agkistrodon, Trimeresurus, Crotalus (family Viperidae), Notechis Elapidae). All group II PLA2s tested displayed when micromolar range concentrations (8-32 microM). In contrast, I PLA2 notexin devoid activity. Aspartate-49 lysine-49 variants showed comparable effect. Skeletal myotubes, obtained fusion differentiation C2C12 myoblasts, significantly more susceptible action myotoxins than cells, reported be undifferentiated myoblasts under same assay conditions. Cytolytic appears characteristic Viperidae. Bee venom PLA2, III enzyme known myotoxicity, also cytotoxic on being cells. These results suggest that differentiated may represent suitable model target for structural found snake venoms.