Naturally Occurring Low Molecular Weight IgM in Patients with Rheumatoid Arthritis, Systemic Lupus Erythematosus and Macroglobulinemia I. Purification and Immunologic Studies

摘要: A method for purification of low molecular weight IgM (LMW IgM) which utilized salt precipitation, gel filtration, and insoluble immunoadsorbents is described. The yield from pathologic sera containing LMW in the range 15 to 36 mg% was approximately 20%. Antisera prepared against IgM, reduced-alkylated recognized common determinants. Purified seropositive rheumatoid subjects did not have factor activity agglutination tests but demonstrated binding 125I-labeled aggregated IgG. a patient with SLE vasculitis had anti-nuclear anti-native DNA activity. When complexed IgG, anti-IgG were essentially equivalent on basis complement fixation. source function biologic processes remain unknown.