Simultaneous Recovery of DNA and RNA from Formalin-Fixed Paraffin-Embedded Tissue and Application in Epidemiologic Studies

摘要: Analysis of DNA, RNA, and protein extracted from tissue specimens in epidemiologic studies is useful for assessing etiologic heterogeneity, mechanisms carcinogenesis, biomarkers prognosis prediction treatment responses. Fresh-frozen samples may provide optimal quality nucleic acids, but pose multiple logistical considerations, including rapid access to tissues before histopathologic examination specialized equipment freezing, transport, storage; addition, morphology often compromised. In contrast, formalin-fixed paraffin-embedded (FFPE) samples, enormous archives existing specimens, represent a valuable source retrospective biological material research, although presenting different limitations compared with frozen samples. Recent efforts have made progress toward enhancing the utility FFPE molecular analyses, DNA studies, increasingly RNA other macromolecules. Here, we report method that used simultaneously recover appreciable quantity discuss briefly application tumor markers studies. Cancer Epidemiol Biomarkers Prev; 19(4); 973–7. ©2010 AACR.