The root-colonizing endophyte Pirifomospora indica confers drought tolerance in Arabidopsis by stimulating the expression of drought stress-related genes in leaves.

摘要: Piriformospora indica is an endophytic fungus that colonizes the roots of many plant species, including Arabidopsis. We exposed 18-day-old Arabidopsis seedlings, which were either cocultivated with or mock-treated for last 9 days, to mild drought stress 84 h. During first 36 48 h, seedlings continued grow, while uncolonized controls did not. This results in a threefold difference fresh weight and more than twofold chlorophyll content. The photosynthetic efficiency was only slightly reduced colonized (F variable/F maximum [Fv/Fm] at t(0 h) = 0.82 t(36 0.79) severely impaired (Fv/Fm 0.81 (t)(36 0.49) also showed symptoms withering. When 72 h transferred soil, 10% (72 none (84 reached flowering stage produced seeds, 59% 47% flowered seeds. After exposure 3 message levels RESPONSE TO DEHYDRATION 29A, EARLY DEHYDRATION1, ANAC072, DEHYDRATION-RESPONSE ELEMENT BINDING PROTEIN2A, SALT-, AND DROUGHT-INDUCED RING FINGER1, phospholipase Ddelta, CALCINEURIN B-LIKE PROTEIN (CBL)1, CBL-INTERACTING KINASE3, histone acetyltransferase (HAT) upregulated leaves P. indica-colonized seedlings. Uncolonized responded 6 later, increased much less. identified ethylmethane-sulfonate mutant less resistant stress-related genes not presence indica. Thus, confers drought-stress tolerance Arabidopsis, this associated priming expression quite diverse set leaves. Transfer soil again faster stronger upregulation CBL1, HAT indicating response might contribute better survival on soil.