Alternate approaches to repress endogenous microRNA activity in Arabidopsis thaliana.

摘要: MicroRNAs (miRNAs) are an endogenous class of regulatory small RNA (sRNA). In plants, miRNAs processed from short non-protein-coding messenger RNAs (mRNAs) transcribed miRNA genes (MIR genes). Traditionally in the model plant Arabidopsis thaliana (Arabidopsis), functional analysis a gene product has relied on identification corresponding T-DNA insertion knockout mutant large, randomly-mutagenized population. However, because size MIR and presence multiple, highly conserved members most families, it been extremely laborious time consuming to obtain single, or null line. Our recent study published Molecular Plant ( 1) outlines alternate method for characterization action Arabidopsis, termed anti-miRNA technology. Using this approach we demonstrated that expression individual miRNAs, entire can be readily efficiently knocked-down. is addition two previously reported methodologies also allow targeted suppression either all family; these include target mimicry transcriptional silencing (TGS) promoters. All three rely machinery article provide overview technologies discuss their strengths weaknesses inhibiting activity miRNA(s).