Chloroplast-targeted Hsp90 plays essential roles in plastid development and embryogenesis in Arabidopsis possibly linking with VIPP1.
作者： Xianyang Chen , Yinxin Li , Juanjuan Feng , Pengxiang Fan , Ping Jiang
DOI: 10.1111/PPL.12083
关键词: Bimolecular fluorescence complementation 、 Thylakoid 、 Mutant 、 Genetics 、 Biogenesis 、 Gene 、 Cell biology 、 Biology 、 Chloroplast 、 Plastid 、 Arabidopsis
摘要: The Arabidopsis genome contains seven members of Hsp90. Mutations in plastid AtHsp90.5 were reported to cause defects chloroplast development and embryogenesis. However, the exact function has not yet been defined. In this study, albino seedlings found among transformed Arabidopsis, which revealed be co-suppressed plants. accumulation photosynthetic super-complexes albinos was decreased, expression genes involved photosynthesis significantly down-regulated. T-DNA insertion mutants embryo-lethal with embryo arrested at heart stage. Further investigation showed up-regulated siliques 4 days post anthesis (DPA). Confocal microscopy proved located chloroplasts. Plastid plants seriously impaired, few thylakoid membranes observed, indicating involvement biogenesis. interact vesicle-inducing protein plastids 1 (VIPP1) by bimolecular fluorescence complementation system. ratio between VIPP1 oligomers monomers drastically shifted toward oligomeric state. Our study confirmed that is vital for biogenesis evidence also suggested may help disassembly membrane formation and/or maintenance.
sci-hub.se 参考文章(73)
Dimitra Milioni, Polydefkis Hatzopoulos, Genomic organization of hsp90 gene family in Arabidopsis. Plant Molecular Biology. ,vol. 35, pp. 955- 961 ,(1997) , 10.1023/A:1005874521528
Robert J. Porra, Wolfram Schafer, Nasr Gad'on, Ingrid Katheder, Gerhart Drews, Hugo Scheer, Origin of the Two Carbonyl Oxygens of Bacteriochlorophyll a FEBS Journal. ,vol. 239, pp. 85- 92 ,(1996) , 10.1111/J.1432-1033.1996.0085U.X
Shari M. Lo, Steven M. Theg, Role of Vesicle‐Inducing Protein in Plastids 1 in cpTat transport at the thylakoid Plant Journal. ,vol. 71, pp. 656- 668 ,(2012) , 10.1111/J.1365-313X.2012.05020.X
Hervé Vaucheret, Christophe Béclin, Taline Elmayan, Frank Feuerbach, Christian Godon, Jean-Benoit Morel, Philippe Mourrain, Jean-Christophe Palauqui, Samantha Vernhettes, Transgene-induced gene silencing in plants. Plant Journal. ,vol. 16, pp. 651- 659 ,(1998) , 10.1046/J.1365-313X.1998.00337.X
Sabina Kovacheva, Jocelyn Bédard, Anthony Wardle, Ramesh Patel, Paul Jarvis, Further in vivo studies on the role of the molecular chaperone, Hsp93, in plastid protein import. Plant Journal. ,vol. 50, pp. 364- 379 ,(2007) , 10.1111/J.1365-313X.2007.03060.X
Lan-Xin Shi, Steven M. Theg, The motors of protein import into chloroplasts. Plant Signaling & Behavior. ,vol. 6, pp. 1397- 1401 ,(2011) , 10.4161/PSB.6.9.16916
Hongmiao Song, Pengxiang Fan, Yinxin Li, Overexpression of Organellar and Cytosolic AtHSP90 in Arabidopsis thaliana Impairs Plant Tolerance to Oxidative Stress Plant Molecular Biology Reporter. ,vol. 27, pp. 342- 349 ,(2009) , 10.1007/S11105-009-0091-6
Barry J. Pogson, Verónica Albrecht, Genetic Dissection of Chloroplast Biogenesis and Development: An Overview Plant Physiology. ,vol. 155, pp. 1545- 1551 ,(2011) , 10.1104/PP.110.170365
Heinrich Heide, André Nordhues, Friedel Drepper, Sabine Nick, Miriam Schulz-Raffelt, Wolfgang Haehnel, Michael Schroda, Application of quantitative immunoprecipitation combined with knockdown and cross-linking to Chlamydomonas
reveals the presence of vesicle-inducing protein in plastids 1 in a common complex with chloroplast HSP90C PROTEOMICS. ,vol. 9, pp. 3079- 3089 ,(2009) , 10.1002/PMIC.200800872
Alicia J. Manfre, Lea M. Lanni, William R. Marcotte, The Arabidopsis Group 1 LATE EMBRYOGENESIS ABUNDANT Protein ATEM6 Is Required for Normal Seed Development Plant Physiology. ,vol. 140, pp. 140- 149 ,(2006) , 10.1104/PP.105.072967