Evaluation of the Nutrient-Sensing mTOR Pathway
作者： Sungki Hong , Aristotle M. Mannan , Ken Inoki
DOI: 10.1007/978-1-61779-430-8_3
关键词: MAP kinase kinase kinase 、 Chemistry 、 mTORC2 、 PI3K/AKT/mTOR pathway 、 Kinase activity 、 Protein kinase B 、 P70-S6 Kinase 1 、 Cell biology 、 RPTOR 、 ASK1
摘要: mTOR, an evolutionarily conserved Ser/Thr protein kinase, belongs to the PI3K-related kinase family, which also includes DNA-PKcs, ATM, and ATR. Although other family members have been shown secure genomic integrity by sensing DNA damage related stresses, mTOR is known function as a nutrient growth factor sensor. catalytic subunit of two distinct multiprotein complexes complex 1 (mTORC1) 2 (mTORC2). In response availability, these regulate variety cellular processes, such cell growth, proliferation, survival modulating downstream effectors, S6K1, 4EBP1, AKT. Therefore, evaluation activity has clear readout in order monitor physiological status cells environmental cues. Here, we present current techniques for assessment different experimental settings.
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Dos D Sarbassov, Siraj M Ali, Shomit Sengupta, Joon-Ho Sheen, Peggy P Hsu, Alex F Bagley, Andrew L Markhard, David M Sabatini, None, Prolonged rapamycin treatment inhibits mTORC2 assembly and Akt/PKB. Molecular Cell. ,vol. 22, pp. 159- 168 ,(2006) , 10.1016/J.MOLCEL.2006.03.029
Tobias Schmelzle, Michael N Hall, TOR, a Central Controller of Cell Growth Cell. ,vol. 103, pp. 253- 262 ,(2000) , 10.1016/S0092-8674(00)00117-3
Eunjung Kim, Pankuri Goraksha-Hicks, Li Li, Thomas P. Neufeld, Kun-Liang Guan, Regulation of TORC1 by Rag GTPases in nutrient response. Nature Cell Biology. ,vol. 10, pp. 935- 945 ,(2008) , 10.1038/NCB1753
Do-Hyung Kim, Dos D. Sarbassov, Siraj M. Ali, Jessie E. King, Robert R. Latek, Hediye Erdjument-Bromage, Paul Tempst, David M. Sabatini, mTOR Interacts with Raptor to Form a Nutrient-Sensitive Complex that Signals to the Cell Growth Machinery Cell. ,vol. 110, pp. 163- 175 ,(2002) , 10.1016/S0092-8674(02)00808-5
J. Choi, J. Chen, S. L. Schreiber, J. Clardy, Structure of the FKBP12-rapamycin complex interacting with the binding domain of human FRAP. Science. ,vol. 273, pp. 239- 242 ,(1996) , 10.1126/SCIENCE.273.5272.239
Tatsuhiro Sato, Akio Nakashima, Lea Guo, Fuyuhiko Tamanoi, Specific activation of mTORC1 by RHEB G-protein in vitro involves enhanced recruitment of its substrate protein Journal of Biological Chemistry. ,vol. 284, pp. 12783- 12791 ,(2009) , 10.1074/JBC.M809207200
Li Yu, Christina K. McPhee, Lixin Zheng, Gonzalo A. Mardones, Yueguang Rong, Junya Peng, Na Mi, Ying Zhao, Zhihua Liu, Fengyi Wan, Dale W. Hailey, Viola Oorschot, Judith Klumperman, Eric H. Baehrecke, Michael J. Lenardo, Termination of autophagy and reformation of lysosomes regulated by mTOR Nature. ,vol. 465, pp. 942- 946 ,(2010) , 10.1038/NATURE09076
Dos D Sarbassov, David A Guertin, Siraj M Ali, David M Sabatini, Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex Science. ,vol. 307, pp. 1098- 1101 ,(2006) , 10.1126/SCIENCE.1106148
Y. Sancak, T. R. Peterson, Y. D. Shaul, R. A. Lindquist, C. C. Thoreen, L. Bar-Peled, D. M. Sabatini, The Rag GTPases Bind Raptor and Mediate Amino Acid Signaling to mTORC1 Science. ,vol. 320, pp. 1496- 1501 ,(2008) , 10.1126/SCIENCE.1157535
Yasemin Sancak, Liron Bar-Peled, Roberto Zoncu, Andrew L. Markhard, Shigeyuki Nada, David M. Sabatini, Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids Cell. ,vol. 141, pp. 290- 303 ,(2010) , 10.1016/J.CELL.2010.02.024