Biophysical characterization of a protein for structure comparison: methods for identifying insulin structural changes

摘要: Although protein structure has been studied for many decades it remains the case that we cannot state with confidence whether two samples have same molecular structure, particularly in solution. The increasing number of biosimilar biopharmaceutical drugs are being tested means this is not an academic exercise. In work consider how four well-established techniques: dynamic light scattering (DLS), circular dichroism (CD), nuclear magnetic resonance spectroscopy (NMR), and modelling can be combined to provide information about supposedly well-understood insulin. A goal was establish a systematic detecting differences between insulin as function pH, temperature, presence or absence zinc, all which known change oligomerisation affect structure. We used recently developed Secondary Structure Neural Network (SSNN) algorithm facilitate analysis CD spectra.