High-performance liquid chromatography—fluorometric and chemiluminometric detection of biogenic amines and other neurotransmitters

摘要: Publisher Summary This chapter focuses on the use of high-performance liquid chromatography (HPLC)–fluorometric and HPLC–chemiluminometric. HPLC is most desirable method for separation neurotransmitters from other substances. The also discusses isolation purification biogenic amines, acetylcholine, histamine, catecholamines, serotonin, which are basic amines. Their properties their chemical structures different. describes materials columns, phases, conditions elution. It extraction, separation, detection neurotransmitters. Substance P, thyrotropin-releasing hormone, luteinizing hormone releasing neurotensin, somatostatin, enkephalins, β-endorphin recognized as neuropeptides or possible neuropeptides. There not many reports HPLC–fluorometric determination neuropeptides, although there publications peptides endocrine tissues brain. usually done by absorption spectrometry.